Counting cells using a hemocytometer
2 Counting cells using a hemocytometer Contents – Preparing the hemocytometer – Preparing the cell suspension – Counting – Viability Preparing the hemocytometer 1. If using a glass hemocytometer and coverslip, clean with alcohol before use.
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4 Figure 1b. ChIP performed with an antibody specific for the RNA polymerase II CTD repeat phosphorylated at S5 (ab5131). The immunoprecipitated DNA was analyzed with primers and probes for the active GAPDH locus (promoter proximal), the inactive
Discover more 1at abcam.com of Differentiation of 3T3-L1 cells into adipocyte-like cells This protocol outlines how to chemically induce the differentiation of 3T3-L1 cells …
1 Sample Quantitative Research Proposal Published by Permission of the Author Dissertation Proposal Christina Ross PhD(c) Topic: Energy Medicine INTRODUCTION
Cell Cycle Analysis DNA Staining by propidium iodide Materials: (Make sure buffers are ice cold as –4°C) 1. 12 X 75 mm polystyrene test tubes.
are preferable, i.e., when the basal efferocytosis is ~10%. If assaying for inhibition of efferocytosis, longer times are better, i.e., when the basal efferocytosis is ~ 30%.
2. Add a large stir bar and autoclave on a liquid cycle for 30 to 40 minutes. 3. Cool to approximately 60 °C while stirring. 4. Using aseptic techniques, add the following stock solutions